Journal:

Article Title: Phospholipase D2 Is Localized to the Rims of the Golgi Apparatus in Mammalian Cells

doi: 10.1091/mbc.02-04-0059

Figure Lengend Snippet: PLD2 localization with different organelles in rat NRK cells. Cells were prepared for immunofluorescence microscopy and incubated with rabbit PLD2 antibody PLD2-27 (A, D, G, and J) and costained with monoclonal antibodies to the ER marker BiP (B); the transferrin receptor (E), a marker of early endosomes and the plasma membrane; and lgp120 (H), a late endosome/lysosomal marker. (K) Cells were stained with caveolin-1, a marker for caveoli; the arrow corresponds to caveolin-1 localized to the plasma membrane. Images were merged to determine overlap between PLD2 (red) and the respective marker proteins (green; C, F, I, and L). Areas of maximal overlap are yellow. (L) Inset, enlargement of the region of overlap between PLD2 and caveolin-1 in the perinuclear Golgi apparatus. The arrow indicates areas where PLD2 puncta (red) either overlap completely with caveolin-1 (green) or are directly adjacent. Images are from projected Z-series. Bars, 10 μm.

Article Snippet: Purified monoclonal anti-rat transferrin receptor antibodies were purchased from Cedarlane Laboratories (Hornby, Ontario, Canada).

Techniques: Immunofluorescence, Microscopy, Incubation, Marker, Staining

Journal: Journal of Virology

Article Title: Dynamics of Virus-Specific Memory B Cells and Plasmablasts following Viral Infection of the Central Nervous System

doi: 10.1128/JVI.00875-18

Figure Lengend Snippet: Virus-specific tdTomato + cells are concentrated in focal white matter tracks along the spinal cord, but rarely form clusters. Representative fluorescent immunohistochemical images of longitudinal spinal cord sections at day 28 p.i. stained for basement membrane laminin (green), nuclear stain DAPI (blue), and endogenous tdTomato expression (red), taken at ×40 magnification from projected Z-stacks of 6.69 μm in depth. The left image illustrates the representative scattered distribution of tdTomato + cells, while the right image depicts a rare cluster of tdTomato + cells. Images are representative of the results for 2 mice. Scale bar = 50 μm.

Article Snippet: Spinal cords were stained as described above using rabbit anti-mouse laminin polyclonal Ab (Cedarlane, Burlington, NC) overnight at 4°C and Alexa Fluor 488 goat anti-hamster Ab (Life Technologies, Grand Island, NY) for 1 h at room temperature.

Techniques: Virus, Immunohistochemical staining, Staining, Membrane, Expressing